| 倍率与分辨率关系汇总 | |||||||
| 类型 | 倍率 |
NA
[平场消色差(PL ACH)]
|
分辨率(um)
0.5*0.55/NA
|
NA
[平场半复消(PL FL)]
|
分辨率(um)
0.5*0.55/NA
|
NA
[平场复消(PL APO)]
|
分辨率(um)
0.5*0.55/NA
|
| 体视 | 0.7 | 0.03 | 9.167 | - | - | - | - |
| 1 | 0.035 | 7.857 | - | - | - | - | |
| 1.5 | 0.04 | 6.875 | - | - | - | - | |
| 2 | 0.045 | 6.111 | - | - | - | - | |
| 2.5 | 0.05 | 5.500 | - | - | - | - | |
| 3 | 0.055 | 5.000 | - | - | - | - | |
| 3.5 | 0.06 | 4.583 | - | - | - | - | |
| 4 | 0.065 | 4.231 | - | - | - | - | |
| 4.5 | 0.07 | 3.929 | - | - | - | - | |
| 生物 | 1 | 0.03 | 9.167 | - | - | - | - |
| 1.6 | 0.04 | 6.875 | - | - | - | - | |
| 2 | 0.05 | 5.500 | 0.070 | 3.929 | 0.080 | 3.438 | |
| 2.5 | 0.07 | 3.929 | - | - | - | - | |
| 4 | 0.10 | 2.750 | 0.130 | 2.115 | 0.160 | 1.719 | |
| 10 | 0.25 | 1.100 | 0.300 | 0.917 | 0.320 | 0.859 | |
| 20/25 | 0.40 | 0.688 | 0.500 | 0.550 | 0.650 | 0.423 | |
| 40 | 0.65 | 0.423 | 0.750 | 0.367 | 0.800 | 0.344 | |
| 50 | 0.65 | 0.423 | 0.750 | 0.367 | 0.800 | 0.344 | |
| 60/63 | 0.65 | 0.423 | 0.850 | 0.324 | 0.900 | 0.306 | |
| 80/100 | 0.80 | 0.344 | 0.850 | 0.324 | 0.900 | 0.306 | |
| 100浸液 | 1.25 | 0.220 | 1.250 | 0.220 | 1.300 | 0.212 | |
| 物镜的数值孔径名义值不应小于下表的规定(引用GBT2609-2015 显微镜 物镜) | ||||||
| 放大率 | 数值孔径(NA) | |||||
| 消色差物镜 | 半复消色差物镜 | 复消色差物镜 | 平场消色差物镜 | 平场半复消色差物镜 | 平场复消色差物镜 | |
| ACH | FL | APO | PL ACH | PL FL | PL APO | |
| 1X | - | - | - | 0.03 | - | - |
| 1.6X | - | - | - | 0.04 | - | - |
| 2X | - | 0.07 | 0.08 | 0.05 | 0.07 | 0.08 |
| 2.5X | - | - | - | 0.07 | - | - |
| 4X | 0.10 | 0.13 | 0.16 | 0.10 | 0.13 | 0.16 |
| 10X | 0.25 | 0.30 | 0.30 | 0.25 | 0.30 | 0.32 |
| 20X/25X | 0.35 | 0.55 | 0.60 | 0.40 | 0.50 | 0.65 |
| 40X | 0.65 | 0.75 | 0.80 | 0.65 | 0.75 | 0.80 |
| 50X | - | 0.75 | 0.80 | 0.65 | 0.75 | 0.80 |
| 60X/63X | 0.80 | 0.85 | 0.90 | 0.65 | 0.85 | 0.90 |
| 80X/100X | - | 0.85 | 0.90 | 0.80 | 0.85 | 0.90 |
| 100X浸液 | 1.25 | 1.25 | 1.30 | 1.25 | 1.25 | 1.30 |